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  Indian J Med Microbiol
 

Figure 1: Overexpression of Snail induces the epithelial–mesenchymal transition in A549 cells. A549-Snail cells, engineered to stably express Snail-HA under the control of a doxycycline (Dox)-inducible promoter, were grown in the absence or presence of 2 μg/ml Dox for 2 days. (a) The levels of Snail-HA, E-cadherin, N-cadherin, and GAPDH proteins were measured using Western blotting. (b) The levels of Snail-HA (red) and E-cadherin (green) were visualized using immunohistochemistry, and the nuclei were visualized using 4′,6-diamidino-2-phenylindole staining (blue). (c) The number of migratory cells was measured using Boyden chamber assay. ****P < 0.0001 (t-test). (d) A549-Snail cells were grown in the absence or presence of 2 μg/ml Dox for the indicated time, and the growth kinetics of the cells were measured using cell counting. ****P < 0.0001 (t-test)

Figure 1: Overexpression of Snail induces the epithelial–mesenchymal transition in A549 cells. A549-Snail cells, engineered to stably express Snail-HA under the control of a doxycycline (Dox)-inducible promoter, were grown in the absence or presence of 2 μg/ml Dox for 2 days. (a) The levels of Snail-HA, E-cadherin, N-cadherin, and GAPDH proteins were measured using Western blotting. (b) The levels of Snail-HA (red) and E-cadherin (green) were visualized using immunohistochemistry, and the nuclei were visualized using 4′,6-diamidino-2-phenylindole staining (blue). (c) The number of migratory cells was measured using Boyden chamber assay. ****<i>P</i> < 0.0001 (<i>t</i>-test). (d) A549-Snail cells were grown in the absence or presence of 2 μg/ml Dox for the indicated time, and the growth kinetics of the cells were measured using cell counting. ****<i>P</i> < 0.0001 (<i>t</i>-test)