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  Indian J Med Microbiol
 

Figure 3: Snail selectively suppresses the expression of glycine decarboxylase. (a) Two clones of A549-Snail cells (clone #1 and #8) were grown in the absence (white bars) or presence (black bars) of 2 μg/ml Dox for 2 days. The levels of SNAI1, GAPDH, glycine decarboxylase, AMT, DLD, and GCSH mRNAs were measured using quantitative reverse transcription-polymerase chain reaction. The result is expressed as fold change relative to the level of the uninduced control. (b) A549-Snail or A549 parent cells were grown in the absence or presence of 2 mg/ml Dox for the indicated time. The levels of Snail-HA, glycine decarboxylase, AMT, DLD, and GAPDH proteins were measured using Western blotting. ****P < 0.0001 (t-test)

Figure 3: Snail selectively suppresses the expression of glycine decarboxylase. (a) Two clones of A549-Snail cells (clone #1 and #8) were grown in the absence (white bars) or presence (black bars) of 2 μg/ml Dox for 2 days. The levels of SNAI1, GAPDH, glycine decarboxylase, AMT, DLD, and GCSH mRNAs were measured using quantitative reverse transcription-polymerase chain reaction. The result is expressed as fold change relative to the level of the uninduced control. (b) A549-Snail or A549 parent cells were grown in the absence or presence of 2 mg/ml Dox for the indicated time. The levels of Snail-HA, glycine decarboxylase, AMT, DLD, and GAPDH proteins were measured using Western blotting. ****<i>P</i> < 0.0001 (<i>t</i>-test)