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  Indian J Med Microbiol
 

Figure 4: Transforming growth factor-b treatment led to a concomitant upregulation of Snail and downregulation of glycine decarboxylase expression in A549 cells. A549 cells were treated with the indicated amounts of transforming growth factor-b for overnight. (a) The levels of SNAI1, CDH1, glycine decarboxylase, AMT, GCSH, and DLD mRNAs were measured using quantitative reverse transcription-polymerase chain reaction. The result is expressed as fold change relative to the level of untreated control. *P < 0.05, ***P < 0.001, ****P < 0.0001 (t-test). (b) The levels of Snail, E-cadherin, glycine decarboxylase, and GAPDH proteins were measured using Western blotting

Figure 4: Transforming growth factor-b treatment led to a concomitant upregulation of Snail and downregulation of glycine decarboxylase expression in A549 cells. A549 cells were treated with the indicated amounts of transforming growth factor-b for overnight. (a) The levels of SNAI1, CDH1, glycine decarboxylase, AMT, GCSH, and DLD mRNAs were measured using quantitative reverse transcription-polymerase chain reaction. The result is expressed as fold change relative to the level of untreated control. *<i>P</i> < 0.05, ***<i>P</i> < 0.001, ****<i>P</i> < 0.0001 (<i>t</i>-test). (b) The levels of Snail, E-cadherin, glycine decarboxylase, and GAPDH proteins were measured using Western blotting