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  Indian J Med Microbiol
 

Figure 5: Snail suppresses the promoter of glycine decarboxylase gene by binding to an evolutionarily conserved proximal E-box motif. (a) glycine decarboxylase genomic sequences at the nucleotide positions from −43 to −2 (top, aligned with the indicated rodent sequences) and from −322 to −281 are exhibited. The E-box motifs are highlighted in red. (b) A549-Snail cells were grown in the presence of 2 mg/ml Dox for 2 days. The binding of Snail-HA to the promoters of glycine decarboxylase, CDH1, and GAPDH were measured using chromatin immunoprecipitation with the anti-HA antibody followed by polymerase chain reaction amplification with the gene-specific primers. The amplified products were visualized using agarose gel electrophoresis, and the sizes of each individual amplicon are labeled at the right. (c) A schematic representation of the luciferase reporter constructs. (d) HEK293 cells were transfected with the indicated plasmids for 2 days, and the luciferase activities of the cells were measured. *P < 0.05, **P < 0.01, ***P < 0.001 (t-test). (e) A549-Snail cells were transfected with the indicated reporter constructs and then grown in the absence (black bars) and presence (white bars) of 2 mg/ml Dox for 2 days, and the luciferase activities of the cells were subsequently measured. *P < 0.05 (t-test)

Figure 5: Snail suppresses the promoter of glycine decarboxylase gene by binding to an evolutionarily conserved proximal E-box motif. (a) glycine decarboxylase genomic sequences at the nucleotide positions from −43 to −2 (top, aligned with the indicated rodent sequences) and from −322 to −281 are exhibited. The E-box motifs are highlighted in red. (b) A549-Snail cells were grown in the presence of 2 mg/ml Dox for 2 days. The binding of Snail-HA to the promoters of glycine decarboxylase, CDH1, and GAPDH were measured using chromatin immunoprecipitation with the anti-HA antibody followed by polymerase chain reaction amplification with the gene-specific primers. The amplified products were visualized using agarose gel electrophoresis, and the sizes of each individual amplicon are labeled at the right. (c) A schematic representation of the luciferase reporter constructs. (d) HEK293 cells were transfected with the indicated plasmids for 2 days, and the luciferase activities of the cells were measured. *<i>P</i> < 0.05, **<i>P</i> < 0.01, ***<i>P</i> < 0.001 (<i>t</i>-test). (e) A549-Snail cells were transfected with the indicated reporter constructs and then grown in the absence (black bars) and presence (white bars) of 2 mg/ml Dox for 2 days, and the luciferase activities of the cells were subsequently measured. *<i>P</i> < 0.05 (<i>t</i>-test)